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My little babies are not ready to be inoculated...
That adds a whole week to my schedule. They have only four true leaves, and I have to wait until they have at least 6, because if not, the infection can delay even more than 15 days... (that because the god damn virus needs short days (8hlight-16h darkness) and a STABLE 23ºC temperature and blah blah blah)
What calms me is that I have everything (Viral clones and gold particles and growth chamber) ready and just when they reach the appropriate size and developmental stage, I'll be bombarding them right away, because I NEED THOSE RESULTS NOW! and the infection is only the first step, I need to take samples of all three lines to make Real-time PCR assays, Gus stainings and complementations...
Jesus!
And the news are: The NPK1-Salk is not really a mutant line, as the insertional homozygous are lethal, I cannot segregate a truly mutant line.
So I have to order a new line from ABRC. :S NPK1 is giving me headaches, because the promoter:reporter gus expressing lines do not express said gene further than in T1 population, as the gus gene and Km gene seem to be silenced, ergo, I'm transforming AGAIN (Three times with this) to have seedlings to work with. I HATE FLORAL DIP! ¬____¬ is so messy... but I prefer that than vacuuming or tissue-culture.
anyway I'm sorry about that lab rant, If you got dizzy for reading this, my apologies.
And.... a music recommendation: Kaiser Chiefs. I'm IN ENDLESS LOVE with them. (Lovely British accents, superb music, emo, despaired lyrics...*faints again*)
Edit: hooooo!! No lab meeting today!! Yahoo!!!
That adds a whole week to my schedule. They have only four true leaves, and I have to wait until they have at least 6, because if not, the infection can delay even more than 15 days... (that because the god damn virus needs short days (8hlight-16h darkness) and a STABLE 23ºC temperature and blah blah blah)
What calms me is that I have everything (Viral clones and gold particles and growth chamber) ready and just when they reach the appropriate size and developmental stage, I'll be bombarding them right away, because I NEED THOSE RESULTS NOW! and the infection is only the first step, I need to take samples of all three lines to make Real-time PCR assays, Gus stainings and complementations...
Jesus!
And the news are: The NPK1-Salk is not really a mutant line, as the insertional homozygous are lethal, I cannot segregate a truly mutant line.
So I have to order a new line from ABRC. :S NPK1 is giving me headaches, because the promoter:reporter gus expressing lines do not express said gene further than in T1 population, as the gus gene and Km gene seem to be silenced, ergo, I'm transforming AGAIN (Three times with this) to have seedlings to work with. I HATE FLORAL DIP! ¬____¬ is so messy... but I prefer that than vacuuming or tissue-culture.
anyway I'm sorry about that lab rant, If you got dizzy for reading this, my apologies.
And.... a music recommendation: Kaiser Chiefs. I'm IN ENDLESS LOVE with them. (Lovely British accents, superb music, emo, despaired lyrics...*faints again*)
Edit: hooooo!! No lab meeting today!! Yahoo!!!
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From:
no subject
no lab meeting! :D
I'm not 100% sure but I think I don't have a meeting at work tomorrow either ^___^
From:
no subject
So I'll be able to prepare the article I'm supposed to explain this wednesday! ^____^
Yay for free time for both of us!
From:
no subject
you lucky... you! *grumbles*
From:
no subject
I was mistaken. I *did* have to go to the meeting, and what's worse, now I have another one on Friday :'(
From:
no subject
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no subject
From:
no subject
thanks! ^______^
(I just hate when every part of me smells like Agrobacterium^)
From:
no subject
From:
no subject
I forgot that I promised to hide that under a cut
From:
no subject
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no subject
Whatever you want me to explain, I'm here ^^
From:
no subject
Also, my caco-2 cells last 4 days in growing and making a monolayer, after that, they last 21 days on diferenciation and after that the permeability assay last no more than 2 hours... taking care of them for weeks and the assay lastes 120 minutes!!
The same story for MDCK-II cells, 4 days growing, 3 days diferenciating and a simple assay of 2 hours.
From:
no subject
I definitely do not envy you. I'm doing fine with my plants, thanks xDDD
*hug*